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      人多發性骨髓瘤細胞

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      產品名稱: 人多發性骨髓瘤細胞
      產品型號: CCL-155
      產品廠商: 美國標準生物品收藏中心(ATCC)
      產品文檔: 無相關文檔


      簡單介紹

      CCL-155 RPMI 8226 人多發性骨髓瘤細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞,細胞庫管理規范,提供 *優培養條件,


      人多發性骨髓瘤細胞 的詳細介紹
      CCL-155 RPMI 8226 人多發性骨髓瘤細胞
      ATCC® Number: CCL-155?
      Designations: RPMI 8226
      Isotype: lambda light chain
      Biosafety Level: 1
      Shipped: frozen
      Medium & Serum: See Propagation
      Growth Properties: suspension
      Organism: Homo sapiens (human)
      Morphology: lymphoblast


      Source: Organ: peripheral blood
      Disease: plasmacytoma; myeloma
      Cell Type: B lymphocyte;
      Cellular Products: immunoglobulin light chain
      Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

      Isolation: Isolation date: June, 1966
      Applications: transfection host
      Virus Susceptibility: Human poliovirus 1
      Herpes simplex virus
      Vaccinia virus
      Vesicular stomatitis virus
      Antigen Expression: HLA Aw19, B15, B37, Cw2; CD19 -; CD20 -; CD28 +; CD38 +; CD49e +
      DNA Profile (STR): Amelogenin: X,Y
      CSF1PO: 12
      D13S317: 11
      D16S539: 9
      D5S818: 11,13
      D7S820: 9,10
      THO1: 8
      TPOX: 8,11
      vWA: 16,18
      Cytogenetic Analysis: Unstable karyotype in triploid range of 68-70 chromosomes. Two large marker chromosomes with terminal centromeres.
      Isoenzymes: G6PD, A
      Age: 61 years
      Gender: male
      Comments: There is no evidence of heavy chain production (cytoplasmic or secreted).
      Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
      Atmosphere: air, 95%; carbon dioxide (CO2), 5%
      Temperature: 37.0°C
      Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml.Do not allow the cell density to exceed 2 to 3 X 10(6) cells/ml.
      Interval: Maintain cell density between 5 X 10(5) and 2 X 10(6) viable cells/ml.
      Medium Renewal: Every 2 to 3 days
      Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
      Storage temperature: liquid nitrogen vapor phase
      Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
      recommended serum:ATCC 30-2020
      purified DNA:ATCC CCL-155D
      References: 1030: Matsuoka Y, et al. Production of free light chains of immunoglobulin by a hematopoietic cell line derived from a patient with multiple myeloma. Proc. Soc. Exp. Biol. Med. 125: 1246-1250, 1967. PubMed: 6042436
      26017: Moore GE, Kitamura H. Cell line derived from patient with myeloma. N.Y. State J. Med. 68: 2054-2060, 1968. PubMed: 5243066
      43268: Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375

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      細胞系,ATCC細胞,ATCC菌種,細胞系目錄,ATCC,鼠傷寒沙門氏菌,atcc細胞庫,人**瘤細胞,人食管癌細胞,ATCC 19404,ATCC 25922,ATCC 19146,微生物菌種,細胞株,標準菌株,ATCC中國,atcc上海代理,CMCC 菌株,ATCC菌株,細胞目錄,質控菌株,腫瘤細胞株,乳腺癌細胞系,人肝癌細胞系

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