產品[
CRL-1581 Sp2/0-Ag14 小鼠骨髓瘤細胞系
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產品名稱:
CRL-1581 Sp2/0-Ag14 小鼠骨髓瘤細胞系
產品型號:
CRL-1581
產品廠商:
美國標準生物品收藏中心（ATCC）
產品文檔:
無相關文檔
簡單介紹
CRL-1581 Sp2/0-Ag14 小鼠骨髓瘤細胞系，ATCC 細胞|細胞系|細胞株|腫瘤細胞株|細胞|貼壁細胞|懸浮細胞|；細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和培養條件
CRL-1581 Sp2/0-Ag14 小鼠骨髓瘤細胞系
的詳細介紹
CRL-1581 Sp2/0-Ag14 小鼠骨髓瘤細胞系
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CRL-1581™ |
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$268.00 |
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Sp2/0-Ag14 |
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G Kohler |
| Biosafety Level: |
1 |
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frozen |
| Medium & Serum: |
See Propagation |
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suspension |
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Mus musculus (B cell); Mus musculus (myeloma) (mouse (B cell); mouse (myeloma)) |
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lymphoblast
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Organ: spleen
Strain: BALB/c
Cell Type: hybridoma: B lymphocyte; |
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
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fusion partner for production of somatic cell hybrids |
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H-2d |
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C |
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Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Maintain cell density between 5 X 10 (4) and 5 X 10 (5) viable cells/ml. Some cells can attach and be transferred by shaking them loose.
Medium Renewal: Add fresh medium every 2 to 4 days (depending on cell density) |
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Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase |
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
derivative:ATCC CRL-1581.1 |
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906: Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126: 317-321, 1981. PubMed: 6935293
1077: Shulman M, et al. A better cell line for making hybridomas secreting specific antibodies. Nature 276: 269-270, 1978. PubMed: 714156
32373: Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260
33167: Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022 |
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