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F4/80
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S Gordon
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IgG2b
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Biosafety Level:
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1
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frozen
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Medium & Serum:
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See Propagation
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suspension
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Rattus norvegicus (B cell); Mus musculus (myeloma) (rat (B cell); mouse (myeloma))
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lymphoblast
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Cell Type: hybridoma: B lymphocyte;
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immunoglobulin; monoclonal antibody; against mouse macrophage
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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No
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Temperature: 37.0°C
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Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
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1635: Hume DA, et al. The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80. Relationship between macrophages, Langerhans cells, reticular cells, and dendritic cells in lymphoid and hematopoietic organs. J. Exp. Med. 158: 1522-1536, 1983. PubMed: 6355361
1636: Austyn JM, Gordon S. F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur. J. Immunol. 11: 805-815, 1981. PubMed: 7308288
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