產(chǎn)品[
Capan-2 人胰腺癌細(xì)胞
]資料
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產(chǎn)品名稱:
Capan-2 人胰腺癌細(xì)胞
產(chǎn)品型號(hào):
HTB-80
產(chǎn)品廠商:
美國(guó)標(biāo)準(zhǔn)生物品收藏中心(ATCC)
產(chǎn)品文檔:
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簡(jiǎn)單介紹
HTB-79 Capan-2 人胰腺癌細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!
Capan-2 人胰腺癌細(xì)胞
的詳細(xì)介紹
HTB-79 Capan-2 人胰腺癌細(xì)胞
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
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Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
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culture medium 95%; DMSO, 5%
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