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        產品[
      
            CRL-1444 大鼠胸大動脈平滑肌細胞
      
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        產品名稱: 
            CRL-1444 大鼠胸大動脈平滑肌細胞
      
        產品型號:
        A7r5  
      
        產品廠商:
        美國標準生物品收藏中心（ATCC）
      
        產品文檔:
        無相關文檔
        
        
        
        
      
        
    
      
    
      
    
      
    
      
        
      
            簡單介紹
        
      
        
      
            
      CRL-1444 A7r5 大鼠胸大動脈平滑肌細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規范，提供的細胞株背景清楚，提供參考文獻和培養條件!
      
        
      
        
      
        
      
            CRL-1444 大鼠胸大動脈平滑肌細胞
            的詳細介紹
        
      
        
      
            
      
	CRL-1444 A7r5 大鼠胸大動脈平滑肌細胞
	
      
		
			
      
				
					ATCC® Number:  
				
				
					CRL-1444?     
				
				
					Price:  
				
				
					$275.00 
				
			
      
			
      
				
					
      
						
							
      
								
									Designations:  
								
								
									A7r5 
								
							
      
							
      
								
									Depositors:  
								
								
									 W Carlisle  
								
							
      
							
      
								
									Biosafety Level: 
								
								
									1 
								
							
      
							
      
								
									Shipped:  
								
								
									frozen  
								
							
      
							
      
								
									Medium & Serum:  
								
								
									See Propagation 
								
							
      
							
      
								
									Growth Properties: 
								
								
									adherent 
								
							
      
							
      
								
									Organism: 
								
								
									Rattus norvegicus (rat) 
								
							
      
							
      
								
									Morphology: 
								
								
									fibroblast
      
CRL-1444 A7r5 大鼠胸大動脈平滑肌細胞
      
       
								      		
							
      
							
      
								
									Source: 
								
								
									Organ: aorta, thoracic 
      
Strain: DB1X 
      
Tissue: smooth muscle       
								      		
							
      
							
      
								
									Cellular Products: 
								
								
									myokinase; creatine phosphokinase (muscle isoenzyme); myosin 
								
							
      
							
      
								
									Permits/Forms: 
								
								
									In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
								
							
      
							
      
								
									 
								
							
      
							
      
								
									Applications: 
								
								
									transfection host (Roche FuGENE® Transfection Reagents
      
technology from amaxa)       
								      		
							
      
							
      
								
									Age:  
								
								
									embryo  
								
							
      
							
      
								
									Comments: 
								
								
									The cells exhibit an increase in activity of the enzymes myokinase and creatine phosphokinase (CPK) as the culture reaches stationary phase. 
      
Muscle type CPK is synthesized after cell division has ceased.       
								      		
							
      
							
      
								
									Propagation:  
								
								
									ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
      
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
      
Temperature: 37.0°C       
								      		
							
      
							
      
								
									Subculturing:  
								
								
									Protocol:  
									
        
										
      1. 
											Remove and discard culture medium.
										
        2. 
										
      2. 
											Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
										
        3. 
										
      3. 
											Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
        
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
										
        4. 
										
      4. 
											Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
										
        5. 
										
      5. 
											Add appropriate aliquots of the cell suspension to new culture vessels.
										
        6. 
										
      6. 
											Incubate cultures at 37°C.
										
        7. 
									
      

      
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended 
      
Medium Renewal: Every 2 to 3 days       
								      		
							
      
							
      
								
									Preservation:  
								
								
									Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 
      
Storage temperature: liquid nitrogen vapor phase       
								      		
							
      
							
      
								
									Related Products: 
								
								
									Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
      
recommended serum:ATCC 30-2020       
								      		
							
      
							
      
								
									References: 
								
								
									1061: Kimes BW, Brandt BL. Characterization of two putative smooth muscle cell lines from rat thoracic aorta. Exp. Cell Res. 98: 349-366, 1976. PubMed: 943301 
								
							
      
						      
					
      
				      		
			
      
		      
	
      


      
    
      
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