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            SK-NEP-1 人腎母細(xì)胞瘤細(xì)胞
      
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        產(chǎn)品名稱: 
            SK-NEP-1 人腎母細(xì)胞瘤細(xì)胞
      
        產(chǎn)品型號:
        HTB-48
      
        產(chǎn)品廠商:
        美國標(biāo)準(zhǔn)生物品收藏中心（ATCC）
      
        產(chǎn)品文檔:
        無相關(guān)文檔
        
        
        
        
      
        
    
      
    
      
    
      
    
      
        
      
            簡單介紹
        
      
        
      
            
      HTB-48 SK-NEP-1 人腎母細(xì)胞瘤細(xì)胞，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種；細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻和培養(yǎng)條件！
      
        
      
        
      
        
      
            SK-NEP-1 人腎母細(xì)胞瘤細(xì)胞
            的詳細(xì)介紹
        
      
        
      
            HTB-48 SK-NEP-1 人腎母細(xì)胞瘤細(xì)胞
      

      
	
		
      
			
				ATCC® Number:  
			
			
				HTB-48?     
			
			
				Price:  
			
			
				$417.00 
			
		
      
		
      
			
				
      
					
						
      
							
								Designations:  
							
							
								SK-NEP-1 
							
						
      
						
      
							
								Depositors:  
							
							
								 G Trempe, LJ Old  
							
						
      
						
      
							
								Biosafety Level: 
							
							
								1 
							
						
      
						
      
							
								Shipped:  
							
							
								frozen  
							
						
      
						
      
							
								Medium & Serum:  
							
							
								See Propagation 
							
						
      
						
      
							
								Growth Properties: 
							
							
								suspension 
							
						
      
						
      
							
								Organism: 
							
							
								Homo sapiens (human) 
							
						
      
						
      
							
								Morphology: 
							
							
								rounded
      

      
       
							      		
						
      
						
      
							
								Source: 
							
							
								Organ: kidney 
      
Disease: Wilms' tumor 
      
Derived from metastatic site: pleural effusion       
							      		
						
      
						
      
							
								Permits/Forms: 
							
							
								In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
							
						
      
						
      
							
								 
							
						
      
						
      
							
								Restrictions: 
							
							
								The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439. 
							
						
      
						
      
							
								Tumorigenic: 
							
							
								Yes 
							
						
      
						
      
							
								Antigen Expression: 
							
							
								Blood Type A; Rh+ 
							
						
      
						
      
							
								DNA Profile (STR): 
							
							
								Amelogenin: X 
      
CSF1PO: 10 
      
D13S317: 11 
      
D16S539: 11 
      
D5S818: 13 
      
D7S820: 8,10 
      
THO1: 8,9.3 
      
TPOX: 8,11 
      
vWA: 15,19       
							      		
						
      
						
      
							
								Cytogenetic Analysis: 
							
							
								(P12) hypotriploid to hypertriploid (+A1, +A2, +C, +D, +E, +F, +G) with abnormalities including acrocentric fragments, secondary constrictions and large subtelocentric markers 
							
						
      
						
      
							
								Isoenzymes:  
							
							
								AK-1, 1 
      
ES-D, 1 
      
G6PD, B 
      
GLO-I, 2 
      
Me-2, 2 
      
PGM1, 1-2 
      
PGM3, 1       
							      		
						
      
						
      
							
								Age:  
							
							
								25 years  
							
						
      
						
      
							
								Gender:  
							
							
								female  
							
						
      
						
      
							
								Ethnicity:  
							
							
								Caucasian  
							
						
      
						
      
							
								Comments: 
							
							
								Ultrastructural features include few microvilli, junctional complexes, well formed Golgi, mostly smooth ER, lipid droplets, no virus particles. 
							
						
      
						
      
							
								Propagation:  
							
							
								ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
      
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
      
Temperature: 37.0°C       
							      		
						
      
						
      
							
								Subculturing:  
							
							
								Medium Renewal: Every 2 to 4 days 
      
Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 1 X 10 exp5 cells/ml and maintain at between 10 exp5 and 10 exp6 cells/ml.       
							      		
						
      
						
      
							
								Preservation:  
							
							
								Culture medium, 95%; DMSO, 5% 
							
						
      
						
      
							
								References: 
							
							
								21869: . Human tumor cells in vitro. New York: Plenum Press; 1975.
      
22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
      
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
      
24381: Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
      
26322: Orkin SH, et al. Development of homozygosity for chromosome 11p markers in Wilms' tumour. Nature 309: 172-174, 1984. PubMed: 6325937       
							      		
						
      
					      
				
      
			      		
		
      
	      

      

      
    
      
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