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Reh
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Univ. Pennsylvania
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Biosafety Level:
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1
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frozen
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See Propagation
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suspension
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Homo sapiens (human)
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lymphoblast
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Disease: acute lymphocytic leukemia (non-T; non-B)
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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CD3 A (17%) B (17%) C (20%), CD4 (15%), CD10 (55%)
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
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Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
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Freeze medium: 95% (v/v) FBS; 5% (v/v) DMSO
Storage temperature: Liquid nitrogen vapor phase
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22700: Greaves M, Janossy G. Patterns of gene expression and the cellular origins of human leukaemias. Biochim. Biophys. Acta 516: 193-230, 1978. PubMed: 365240
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