CRL-1721 PC-12 大鼠腎上腺嗜鉻細(xì)胞瘤細(xì)胞
ATCC® Number: CRL-1721?
Designations: PC-12
Depositors: B Patterson
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: floating clusters; few scattered lightly attached cells.
Organism: Rattus norvegicus (rat)
Morphology: small irregularly shaped cells
Source: Organ: adrenal gland
Disease: pheochromocytoma
Cellular Products: catecholamines; dopamine; norepinephrine [1163]
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host (Roche FuGENE® Transfection Reagents
technology from amaxa)
Receptors: nerve growth factor (NGF), expressed
Tumorigenic: Yes
Cytogenetic Analysis: 40 chromosomes; 38 autosomes plus XY [1163]
Gender: male
Comments: The PC-12 cell line was derived from a transplantable rat pheochromocytoma. [1163]
The cells respond reversibly to NGF by induction of the neuronal phenotype when plated on Collagen IV coated culture flasks. [1163]
The cells do not synthesize epinephrine. [1163]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
heat-inactivated horse serum to a final concentration of 10%
fetal bovine serum to a final concentration of 5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Volumes used for this protocol are for a 75cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Transfer cell suspension to centrifuge tube. Centrifuge cells at 180 to 225 xg for 8-15 minutes at room temperature.
Remove and discard supernatant leaving cell pellet.
Resuspend the cell pellet in an appropriate volume of fresh medium (about one tenth of the original volume.
Gently aspirate each 5 ml aliquot of cells 4 or 5 times with a new 20 ml syringe outfitted with a 22g (1? in.) needle to break up cell clusters.
Add appropriate aliquots of the cell suspension to new 75 cm2 flask with 10-15 ml fresh growth medium. Seed flask 5 x 10(5) to 1 x 10(6) viable cells/ml or use subcultivation ratio of 1:2 to 1:4.
Place culture vessels in incubator at 37oC Subculture when cell density reaches between 2-4 x 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 48 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
ATCC CRL-1721.1, PC-1
References: 1162: Levi A, et al. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 229: 393-395, 1985. PubMed: 3839317
1163: Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897
22344: Biocca S, et al. A macromolecular structure favouring microtubule assembly in NGF- differentiated pheochromocytoma cells (PC12). EMBO J. 2: 643-648, 1983. PubMed: 6641712
33014: Weber E, et al. Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271: 6963-6971, 1996. PubMed: 8636125
