CRL-1840 OK 負(fù)鼠腎細(xì)胞
ATCC® Number: CRL-1840?
Designations: OK
Depositors: MM Miller
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Didelphis marsupialis virginiana (opossum)
Morphology: epithelial
Source: Organ: kidney, cortex
Tissue: proximal tubule
Disease: normal
Cell Type: epithelial
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Applications: transfection host (Roche FuGENE® Transfection Reagents)
Receptors: alpha 2 adrenergic, expressed
atrial natriuretic peptide (ANP), expressed
serotonin, expressed
parathyroid hormone (PTH), expressed
alpha 2 adrenergic; serotonin; parathyroid hormone (PTH); atrial natriuretic peptide (ANP)
Age:
Gender: female
Comments: This line was originally developed for use as a source of X chromosomes in a study of X inactivation, and was subsequently found to be an excellent cell culture model for the kidney proximal tubule epithelium.
The cells display a variety of receptors in culture, and have been widely used as a model for studying those receptors.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 1599: Yagil C, et al. Insulin binding, internalization, and degradation by a cultured kidney cell line. Am. J. Physiol. 254: E601-E608, 1988. PubMed: 2834959
1601: Cole JA, et al. Regulation of sodium-dependent phosphate transport by parathyroid hormone in opossum kidney cells: adenosine 3', 5'-monophosphate-dependent and -independent mechanisms. Endocrinology 122: 2981-2989, 1988. PubMed: 2836179
1602: Nakai M, et al. Atrial natriuretic factor inhibits phosphate uptake in opossum kidney cells as a model of renal proximal tubules. Biochem. Biophys. Res. Commun. 152: 1416-1420, 1988. PubMed: 2837187
22318: Cheng L, et al. Alpha-2-adrenergic modulation of the parathyroid hormone-inhibition of phosphate uptake in cultured renal (OK) cells. Biochem. Biophys. Res. Commun. 155: 74-82, 1988. PubMed: 2843189
22651: Koyama H, et al. Establishment and characterization of a cell line from the American opossum (Didelphys virginiana). In Vitro 14: 239-246, 1978. PubMed: 566717
23383: . . J. Pharmacol. 244: 571-578, 1988.
